Hotspot

rbcL-accD

LSC · 56347-57069 · 723 bp

Back to Bellevalia

Pi 0.0142

MarkerSeek score 69.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 10
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GATTATCCGTGAAGCTGCCA	CCGGTATGTCCAACAGCATT	50.0	50.0	58.0	58.2	942 bp	0.5	57.6
rbcL-accD_p2	GATTATCCGTGAAGCTGCCA	CTTCCACCGGTATGTCCAAC	50.0	55.0	58.0	58.3	948 bp	0.5	57.5
rbcL-accD_p3	TTATCCGTGAAGCTGCCAAA	CCGGTATGTCCAACAGCATT	45.0	50.0	57.8	58.2	940 bp	0.5	56.5
rbcL-accD_p4	TTATCCGTGAAGCTGCCAAA	CTTCCACCGGTATGTCCAAC	45.0	55.0	57.8	58.3	946 bp	0.5	56.3
rbcL-accD_p5	GATTATCCGTGAAGCTGCCA	AGCCCGCATTTATGTTCTGA	50.0	45.0	58.0	57.6	900 bp	0.5	56.3