Hotspot

trnT-GGU-psbD

LSC · 32201-33215 · 1015 bp

Back to Bellevalia

Pi 0.0198

MarkerSeek score 72.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 19
pi: 0.0198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGATCCCTCCGTAACC	55.0	55.0	58.1	58.1	1118-1157 bp	1.0	84.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGATCCCTCCGTAACC	50.0	55.0	58.1	58.1	1121-1160 bp	1.0	83.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1136-1175 bp	1.0	83.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1139-1178 bp	1.0	83.4
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CGATCCCTCCGTAACCAATC	55.0	55.0	58.1	57.8	1114-1153 bp	1.0	83.4