Hotspot

trnE-UUC-trnT-GGU

LSC · 31756-32128 · 373 bp

Back to Bellevalia

Pi 0.0375
MarkerSeek score 66.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
373
variable_sites
14
pi
0.0375
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGAATCCCCGTTTTCTCCTT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 485 bp 1.0 81.4
trnE-UUC-trnT-GGU_p2 CGAATCCCCGTTTTCTCCTT CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 464 bp 1.0 81.0
trnE-UUC-trnT-GGU_p3 CGAATCCCCGTTTTCTCCTT TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 461 bp 1.0 80.9
trnE-UUC-trnT-GGU_p4 GGAAGTCGAATCCCCGTTTT GAACCGATGACTTACGCCTT 50.0 50.0 58.2 58.0 491 bp 1.0 80.8
trnE-UUC-trnT-GGU_p5 GGAAGTCGAATCCCCGTTTT CCATGGCGTTACTCTACCAC 50.0 55.0 58.2 58.1 470 bp 1.0 80.4