Hotspot

trnS-GCU-trnG-UCC

LSC · 8521-9460 · 940 bp

Back to Bellevalia

Pi 0.0157

MarkerSeek score 57.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 13
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCGAACCCTCGGTACAAGTA	TACCACTAAACTATACCCGC	50.0	45.0	57.8	54.0	945-1033 bp	1.0	51.4
trnS-GCU-trnG-UCC_p2	GGAACGGAGAGAGAGGGATT	TACCACTAAACTATACCCGC	55.0	45.0	58.2	54.0	964-1052 bp	1.0	51.2
trnS-GCU-trnG-UCC_p3	AATCTGCCGCTTTAGTCCAC	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	906-994 bp	1.0	50.9
trnS-GCU-trnG-UCC_p4	CAATCTGCCGCTTTAGTCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	907-995 bp	1.0	50.9
trnS-GCU-trnG-UCC_p5	CGGAGAGAGAGGGATTCGAA	TACCACTAAACTATACCCGC	55.0	45.0	58.3	54.0	960-1048 bp	1.0	50.4