Hotspot

rps16-trnQ-UUG

LSC · 5809-7249 · 1441 bp

Back to Bellevalia

Pi 0.0271
MarkerSeek score 64.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1441
variable_sites
35
pi
0.0271
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TATGTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1392-1524 bp 1.0 85.2
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1382-1514 bp 1.0 84.2
rps16-trnQ-UUG_p3 TATGTCCTTCAAGTCGCACG CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1396-1528 bp 1.0 84.2
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 1386-1518 bp 1.0 83.1
rps16-trnQ-UUG_p5 TATGTCCTTCAAGTCGCACG TACTCGGAGGTTCGAATCCT 50.0 50.0 58.0 57.6 1398-1530 bp 1.0 82.4