Hotspot

ycf1

SSC · 129550-135054 · 5505 bp

Back to Beilschmiedia

Pi 0.0086

MarkerSeek score 52.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5505
variable_sites: 312
pi: 0.0086
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAGAAGAACCAAGATTCCAGG	AAAATTACTAAGACTGGGACTAA	45.5	30.4	57.7	52.8	180-204 bp	1.0	52.0
ycf1_p2	GAGAAGAACCAAGATTCCAGGT	AAAATTACTAAGACTGGGACTAA	45.5	30.4	57.7	52.8	179-203 bp	1.0	52.0
ycf1_p3	GGCACAAATTCTGAGAAGAACC	AAAATTACTAAGACTGGGACTAA	45.5	30.4	57.8	52.8	191-215 bp	1.0	51.9
ycf1_p4	TGCATCATAAAGTGGCACAA	AAAATTACTAAGACTGGGACTAA	40.0	30.4	55.9	52.8	204-228 bp	1.0	51.8
ycf1_p5	AGTGGCACAAATTCTGAGAAGA	AAAATTACTAAGACTGGGACTAA	40.9	30.4	57.9	52.8	194-218 bp	1.0	51.8