Hotspot

clpP

LSC · 75126-77190 · 2065 bp

Back to Beilschmiedia

Pi 0.00223

MarkerSeek score 64.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2065
variable_sites: 31
pi: 0.00223
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGATCGATCCTAACCAGATGA	ACGTTTCCACATCAAAGTGA	42.9	40.0	55.4	55.5	2186-2242 bp	1.0	44.0
clpP_p2	AGATCGATCCTAACCAGATGA	ACGTTTCCACATCAAAGTGAA	42.9	38.1	55.4	56.3	2186-2242 bp	1.0	44.0
clpP_p3	AGATCGATCCTAACCAGATGAT	ACGTTTCCACATCAAAGTGA	40.9	40.0	55.9	55.5	2186-2242 bp	1.0	44.0
clpP_p4	AGATCGATCCTAACCAGATGA	ACGTTTCCACATCAAAGTGAAA	42.9	36.4	55.4	57.0	2186-2242 bp	1.0	44.0
clpP_p5	GATCGATCCTAACCAGATGA	ACGTTTCCACATCAAAGTGA	45.0	40.0	53.8	55.5	2185-2241 bp	1.0	44.0