Hotspot

rps15-ycf1

SSC · 129174-129549 · 376 bp

Back to Beilschmiedia

Pi 0.00921
MarkerSeek score 66.60
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
376
variable_sites
26
pi
0.00921
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TGAAATTCAACAGACCCTTTGT TGGCAGTCGTTTCAGTATGT 36.4 45.0 56.4 57.2 502-516 bp 0.947368 49.5
rps15-ycf1_p2 TGAAATTCAACAGACCCTTTGTT TGGCAGTCGTTTCAGTATGT 34.8 45.0 57.1 57.2 502-516 bp 0.947368 47.0
rps15-ycf1_p3 TTGAAATTCAACAGACCCTTTGT TGGCAGTCGTTTCAGTATGT 34.8 45.0 57.1 57.2 503-517 bp 0.947368 47.0
rps15-ycf1_p4 ACTTGAAATTCAACAGACCCT TGGCAGTCGTTTCAGTATGT 38.1 45.0 55.3 57.2 505-519 bp 0.894737 45.9
rps15-ycf1_p5 ACTTGAAATTCAACAGACCCTT TGGCAGTCGTTTCAGTATGT 36.4 45.0 56.1 57.2 505-519 bp 0.894737 44.2