Hotspot

rpl32-trnL-UAG

SSC · 118531-119895 · 1365 bp

Back to Beilschmiedia

Pi 0.00693

MarkerSeek score 62.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1365
variable_sites: 76
pi: 0.00693
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCGATTCAATTTCCACGGT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1886-1943 bp	0.947368	83.3
rpl32-trnL-UAG_p2	ACCGATTCAATTTCCACGGT	ACTGGGATTGTAGAGCAGGT	45.0	50.0	57.8	58.0	2435-2493 bp	0.894737	81.2
rpl32-trnL-UAG_p3	AAAGGAAAGGGATGTCAGGC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	1462-1516 bp	0.842105	78.4
rpl32-trnL-UAG_p4	ACTCGATCTCGGAGCTTGAT	ACTGGGATTGTAGAGCAGGT	50.0	50.0	58.3	58.0	2607-2666 bp	0.842105	78.0
rpl32-trnL-UAG_p5	AAAGGAAAGGGATGTCAGGC	ACTGGGATTGTAGAGCAGGT	50.0	50.0	57.8	58.0	2011-2066 bp	0.789474	76.3