Hotspot

ndhF-rpl32

SSC · 117179-118374 · 1196 bp

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Pi 0.0106
MarkerSeek score 61.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1196
variable_sites
86
pi
0.0106
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAGCAAAAGGT TTCGACACAAGAGGGAGTCT 50.0 50.0 58.0 58.0 1968-2026 bp 1.0 87.8
ndhF-rpl32_p2 CTGGAAGTGGAGCAAAAGGT CCATTCGACACAAGAGGGAG 50.0 55.0 58.0 58.0 1971-2029 bp 1.0 87.8
ndhF-rpl32_p3 CGGAACAAACAGAAGTCCCA TTCGACACAAGAGGGAGTCT 50.0 50.0 58.0 58.0 2006-2064 bp 1.0 87.6
ndhF-rpl32_p4 CGGAACAAACAGAAGTCCCA CCATTCGACACAAGAGGGAG 50.0 55.0 58.0 58.0 2009-2067 bp 1.0 87.5
ndhF-rpl32_p5 AGGGGAAATTGTGCGGATTT TTCGACACAAGAGGGAGTCT 45.0 50.0 58.1 58.0 2653-2711 bp 1.0 86.1