Hotspot

petA-psbJ

LSC · 67925-69084 · 1160 bp

Back to Beilschmiedia

Pi 0.00925

MarkerSeek score 73.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 43
pi: 0.00925
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1228-1298 bp	1.0	78.2
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGG	45.0	50.0	58.2	56.7	1277-1347 bp	1.0	75.0
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	CTGGTATTCCTGTGATCGGT	45.0	50.0	58.2	56.7	1276-1346 bp	1.0	75.0
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	45.0	47.6	58.2	57.5	1235-1305 bp	1.0	73.3
petA-psbJ_p5	ACGTGTCCAAGGTTTGTTGT	ACTGGTATTCCTGTGATCGGT	45.0	47.6	58.2	58.5	1277-1347 bp	1.0	73.0