Hotspot

trnC-GCA-petN

LSC · 30071-31154 · 1084 bp

Back to Beilschmiedia

Pi 0.00611

MarkerSeek score 64.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 45
pi: 0.00611
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1107-1206 bp	1.0	85.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1051-1150 bp	1.0	85.3
trnC-GCA-petN_p3	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	1033-1132 bp	1.0	84.5
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1168-1267 bp	1.0	83.7
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1112-1211 bp	1.0	83.5