Hotspot

atpH-atpI

LSC · 15735-16653 · 919 bp

Back to Beilschmiedia

Pi 0.00713

MarkerSeek score 61.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 919
variable_sites: 29
pi: 0.00713
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.1	57.9	814-1022 bp	0.947368	82.3
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.9	57.9	872-1080 bp	0.947368	82.1
atpH-atpI_p3	AACGGAAGCAGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	784-992 bp	0.947368	82.0
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	832-1040 bp	0.947368	81.9
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	842-1050 bp	0.947368	81.8