Hotspot

rps16-trnQ-UUG

LSC · 7858-8854 · 997 bp

Back to Beilschmiedia

Pi 0.00343

MarkerSeek score 63.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 997
variable_sites: 28
pi: 0.00343
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GCAAGAATCCACAGCCGATA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1055-1116 bp	1.0	85.1
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1026-1087 bp	1.0	84.4
rps16-trnQ-UUG_p3	GCAAGAATCCACAGCCGATA	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.0	57.8	1059-1120 bp	1.0	84.0
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1030-1091 bp	1.0	83.3
rps16-trnQ-UUG_p5	CCACAGCCGATAATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1047-1108 bp	1.0	82.8