Hotspot

ycf1

SSC · 128469-133817 · 5349 bp

Back to Begonia

Pi 0.0392

MarkerSeek score 73.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5349
variable_sites: 1981
pi: 0.0392
unique_haplotype_count: 112
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTGCCAAGGTTTAAGACGA	ATCCTCCGGATGGGAATCAA	50.0	50.0	58.1	58.2	1482-1551 bp	0.964602	88.2
ycf1_p2	TCGGTGCCAAGGTTTAAGAC	ATCCTCCGGATGGGAATCAA	50.0	50.0	58.1	58.2	1484-1553 bp	0.929204	86.8
ycf1_p3	TCTTTGAGGGCGCATAGAAC	ATCCTCCGGATGGGAATCAA	50.0	50.0	58.0	58.2	920-989 bp	0.893805	86.1
ycf1_p4	GTCTTTGAGGGCGCATAGAA	ATCCTCCGGATGGGAATCAA	50.0	50.0	58.0	58.2	921-990 bp	0.884956	85.8
ycf1_p5	ATCCTCGGACCATTCAGGAA	ATCCTCCGGATGGGAATCAA	50.0	50.0	58.1	58.2	1388-1400 bp	0.115044	50.9