Hotspot

clpP-psbB

LSC · 64058-64536 · 479 bp

Back to Begonia

Pi 0.0284

MarkerSeek score 78.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 176
pi: 0.0284
unique_haplotype_count: 98
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GAACAGAAAGCAAACGACCG	50.0	50.0	58.0	58.0	600-674 bp	0.955752	90.1
clpP-psbB_p2	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	553-627 bp	0.982301	89.4
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CAAACGACCGGGATCATTCA	50.0	50.0	58.0	58.3	590-664 bp	0.982301	89.1
clpP-psbB_p4	CGTCAACCCAAGATGCATCT	GAACAGAAAGCAAACGACCG	50.0	50.0	58.3	58.0	591-665 bp	0.973451	88.7
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	GAACAGAAAGCAAACGACCG	50.0	50.0	57.7	58.0	571-645 bp	0.964602	88.0