Hotspot

rbcL-accD

LSC · 48986-49693 · 708 bp

Back to Begonia

Pi 0.0411

MarkerSeek score 79.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 300
pi: 0.0411
unique_haplotype_count: 107
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	TTGAACCCACAAATGCCTGT	50.0	45.0	58.0	58.2	1310-1502 bp	0.911504	86.5
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	ATAGGAGCCCAAGTACCTGG	50.0	55.0	58.0	58.2	1467-1642 bp	0.256637	60.5
rbcL-accD_p3	CGCTGGTACCGTAGTAGGTA	ATAGGAGCCCAAGTACCTGG	55.0	55.0	58.0	58.2	1780-1955 bp	0.256637	60.4
rbcL-accD_p4	ACTACAATTCGGTGGAGGGA	ATAGGAGCCCAAGTACCTGG	50.0	55.0	58.1	58.2	1564-1739 bp	0.256637	60.3
rbcL-accD_p5	GGGTCTCTCTACCAGGTGTT	ATAGGAGCCCAAGTACCTGG	55.0	55.0	58.1	58.2	1658-1833 bp	0.256637	60.2