Hotspot

ndhC-trnV-UAC

LSC · 43140-43803 · 664 bp

Back to Begonia

Pi 0.0441
MarkerSeek score 81.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
664
variable_sites
310
pi
0.0441
unique_haplotype_count
101
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCATAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 26.1 55.0 52.2 58.1 587-877 bp 1.0 52.0
ndhC-trnV-UAC_p2 TCATATTCATAAAGCAGAAACAT CCGAGAAAGTCTACGGTTCG 26.1 55.0 52.2 58.1 586-876 bp 1.0 52.0
ndhC-trnV-UAC_p3 CCCAGAAAATATCATATTCATAAAGCA CCGAGAAAGTCTACGGTTCG 29.6 55.0 56.4 58.1 597-887 bp 0.99115 51.6
ndhC-trnV-UAC_p4 ACCCAGAAAATATCATATTCATAAAGC CCGAGAAAGTCTACGGTTCG 29.6 55.0 56.4 58.1 598-888 bp 0.99115 51.6
ndhC-trnV-UAC_p5 CCCAGAAAATATCATATTCATAAAGC CCGAGAAAGTCTACGGTTCG 30.8 55.0 54.9 58.1 597-887 bp 0.99115 51.6