Pi
0.0513
MarkerSeek score
79.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | TTGACCCGTTGATCCTTTGG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1280-2018 bp | 0.964602 | 89.8 |
| trnT-UGU-trnL-UAA_p2 | TACCCTTCCGGTTCTGGATT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.0 | 58.0 | 956-1606 bp | 0.823009 | 84.6 |
| trnT-UGU-trnL-UAA_p3 | TACCCTTCCGGTTCTGGATT | ACGCAGTCAACTCCATTTGT | 50.0 | 45.0 | 58.0 | 58.0 | 1177-1825 bp | 0.778761 | 82.6 |
| trnT-UGU-trnL-UAA_p4 | CCGGTTCTGGGCTTTTTACT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1139-1831 bp | 0.495575 | 71.0 |
| trnT-UGU-trnL-UAA_p5 | TCCGGTTCTGGGCTTTTTAC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1140-1832 bp | 0.495575 | 71.0 |