Hotspot

psbZ-trnG-GCC

LSC · 27641-28223 · 583 bp

Back to Begonia

Pi 0.0556

MarkerSeek score 82.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 290
pi: 0.0556
unique_haplotype_count: 106
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	TACCATTCAACTATATCCGC	55.0	40.0	58.0	52.3	470-754 bp	0.964602	50.6
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	TTACCATTCAACTATATCCGC	55.0	38.1	58.0	53.2	471-755 bp	0.964602	50.6
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	TACCATTCAACTATATCCGC	50.0	40.0	57.8	52.3	473-757 bp	0.964602	50.6
psbZ-trnG-GCC_p4	CTTCTCCTGATGGTTGGTCG	TTTACCATTCAACTATATCCGC	55.0	36.4	58.0	54.1	472-756 bp	0.964602	50.6
psbZ-trnG-GCC_p5	GATGGTTGGTCGGGTAACAA	TACCATTCAACTATATCCGC	50.0	40.0	57.8	52.3	462-746 bp	0.902655	48.1