Hotspot

trnE-UUC-trnT-GGU

LSC · 21562-22752 · 1191 bp

Back to Begonia

Pi 0.0425

MarkerSeek score 83.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1191
variable_sites: 534
pi: 0.0425
unique_haplotype_count: 106
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	677-1348 bp	0.99115	90.3
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	639-1310 bp	0.99115	89.5
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	618-1289 bp	0.99115	89.3
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	656-1327 bp	0.946903	88.1
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	653-1324 bp	0.946903	87.9