Hotspot

trnS-UGA

LSC · 38412-38504 · 93 bp

Back to Beesia

Pi 0.0645
MarkerSeek score 74.90
Primer available yes
Label trnS-UGA

Feature Metadata

label_name
trnS-UGA
feature_type
tRNA
region
LSC
length_bp
93
variable_sites
6
pi
0.0645
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA_p1 GCCGGGCGAAACAAATAAAG CGGGTGTTTCTACGGATAGTG 50.0 52.4 58.4 58.2 219-223 bp 1.0 71.1
trnS-UGA_p2 GCCGGGCGAAACAAATAAAG CGGGTGTTTCTACGGATAGT 50.0 50.0 58.4 56.8 219-223 bp 1.0 70.7
trnS-UGA_p3 AGCCGGGCGAAACAAATAAA CGGGTGTTTCTACGGATAGTG 45.0 52.4 58.8 58.2 220-224 bp 1.0 68.0
trnS-UGA_p4 AGCCGGGCGAAACAAATAAA CGGGTGTTTCTACGGATAGT 45.0 50.0 58.8 56.8 220-224 bp 1.0 67.7
trnS-UGA_p5 AGCCGGGCGAAACAAATAA CGGGTGTTTCTACGGATAGTG 47.4 52.4 58.1 58.2 220-224 bp 1.0 65.6