Hotspot

ccsA-ndhD

SSC · 118881-119141 · 261 bp

Back to Beesia

Pi 0.0425
MarkerSeek score 70.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
261
variable_sites
11
pi
0.0425
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CAATTGTGGCTTCTATGGGA TCCGGATTTCGTTCTCTCAC 45.0 50.0 55.4 57.4 441-449 bp 1.0 56.3
ccsA-ndhD_p2 CAATTGTGGCTTCTATGGGA CCGGATTTCGTTCTCTCACT 45.0 50.0 55.4 57.3 440-448 bp 1.0 56.3
ccsA-ndhD_p3 CAATTGTGGCTTCTATGGGAT TCCGGATTTCGTTCTCTCAC 42.9 50.0 56.0 57.4 441-449 bp 1.0 52.9
ccsA-ndhD_p4 CAATTGTGGCTTCTATGGGAT CCGGATTTCGTTCTCTCACT 42.9 50.0 56.0 57.3 440-448 bp 1.0 52.9
ccsA-ndhD_p5 CAATTGTGGCTTCTATGGGA ATCCGGATTTCGTTCTCTCAC 45.0 47.6 55.4 57.8 442-450 bp 1.0 52.2