Hotspot

ndhF-trnL-UAG

SSC · 116574-117702 · 1129 bp

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Pi 0.0186
MarkerSeek score 68.80
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1129
variable_sites
20
pi
0.0186
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 ACGAAAGGTATGATCCACGC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1174-1199 bp 1.0 84.3
ndhF-trnL-UAG_p2 CGAAAGGTATGATCCACGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1173-1198 bp 1.0 84.3
ndhF-trnL-UAG_p3 ACGAAAGGTATGATCCACGC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1175-1200 bp 1.0 84.3
ndhF-trnL-UAG_p4 CTGGAACAAGGATAAGCCCC AGCGTGTCTACCGATTTCAC 55.0 50.0 57.9 58.0 1224-1249 bp 1.0 84.1
ndhF-trnL-UAG_p5 CTGGAACAAGGATAAGCCCC CAGCGTGTCTACCGATTTCA 55.0 50.0 57.9 58.0 1225-1250 bp 1.0 84.1