Hotspot

ycf1-ndhF

SSC · 114017-114329 · 313 bp

Back to Beesia

Pi 0.444
MarkerSeek score 61.30
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
313
variable_sites
20
pi
0.444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.14
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 ACGAAGGGGACTAAACAGGA TGCTCGTTGGATCCATAGGA 50.0 50.0 58.0 58.2 982-1251 bp 1.0 84.6
ycf1-ndhF_p2 CCTTCCCCTTCCCTTTGTTC TGCTCGTTGGATCCATAGGA 55.0 50.0 58.1 58.2 937-1206 bp 1.0 84.2
ycf1-ndhF_p3 TTTAGGCAGAATACCGTCGC TGCTCGTTGGATCCATAGGA 50.0 50.0 58.1 58.2 1097-1366 bp 1.0 84.0
ycf1-ndhF_p4 AGGGGGAAAAGTGAGGAAGA TGCTCGTTGGATCCATAGGA 50.0 50.0 57.9 58.2 1033-1302 bp 1.0 83.6
ycf1-ndhF_p5 GCAGAATACCGTCGCCTATT TGCTCGTTGGATCCATAGGA 50.0 50.0 57.8 58.2 1092-1361 bp 1.0 83.3