Hotspot

rpl16-rps3

LSC · 85780-85950 · 171 bp

Back to Beesia

Pi 0.0247
MarkerSeek score 71.30
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
171
variable_sites
4
pi
0.0247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AAACCAACGAGTCACACACT GGATATTTGCAGGCGAGGAA 45.0 50.0 57.6 58.0 212-220 bp 1.0 81.4
rpl16-rps3_p2 AAACCAACGAGTCACACACT TCGAACTATCTACGGGGCAT 45.0 50.0 57.6 58.0 247-255 bp 1.0 80.9
rpl16-rps3_p3 AAACCAACGAGTCACACACT GTTCGAACTATCTACGGGGC 45.0 55.0 57.6 57.9 249-257 bp 1.0 79.9
rpl16-rps3_p4 AAACCAACGAGTCACACACT TTCGAACTATCTACGGGGCA 45.0 50.0 57.6 58.2 248-256 bp 1.0 79.1
rpl16-rps3_p5 AAACCAACGAGTCACACACT TTGGATATTTGCAGGCGAGG 45.0 50.0 57.6 58.3 214-222 bp 1.0 79.0