Hotspot

rpl14-rpl16

LSC · 84228-84332 · 105 bp

Back to Beesia

Pi 0.07
MarkerSeek score 76.30
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
105
variable_sites
7
pi
0.07
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 GGCGATTACTGGCTCCTATG GCTAGGGATGTGGAACGAAA 55.0 50.0 57.9 57.6 220-229 bp 1.0 80.4
rpl14-rpl16_p2 CATATCGGCGATTACTGGCT GCTAGGGATGTGGAACGAAA 50.0 50.0 57.6 57.6 226-235 bp 1.0 78.0
rpl14-rpl16_p3 GGCGATTACTGGCTCCTATG ATTGCTAGGGATGTGGAACG 55.0 50.0 57.9 57.7 223 bp 0.5 56.7
rpl14-rpl16_p4 AGCATATCGGCGATTACTGG ATTGCTAGGGATGTGGAACG 50.0 50.0 57.6 57.7 231 bp 0.5 54.4
rpl14-rpl16_p5 CATATCGGCGATTACTGGCT ATTGCTAGGGATGTGGAACG 50.0 50.0 57.6 57.7 229 bp 0.5 54.4