Hotspot

trnW-CCA-trnP-UGG

LSC · 69495-69670 · 176 bp

Back to Beesia

Pi 0.0455
MarkerSeek score 78.30
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
176
variable_sites
8
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GAAGAACGGAATCACGCTCT GTCACGGGTTCAAATCCTGT 50.0 50.0 58.0 58.1 290-308 bp 1.0 81.3
trnW-CCA-trnP-UGG_p2 AGAAGAACGGAATCACGCTC GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 302-320 bp 1.0 81.2
trnW-CCA-trnP-UGG_p3 GAAGAACGGAATCACGCTCT GGGTACAAAATGTCACGGGT 50.0 50.0 58.0 58.1 301-319 bp 1.0 81.2
trnW-CCA-trnP-UGG_p4 AGAAGAACGGAATCACGCTC TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 303-321 bp 1.0 81.2
trnW-CCA-trnP-UGG_p5 GAAGAACGGAATCACGCTCT TGGGTACAAAATGTCACGGG 50.0 50.0 58.0 58.1 302-320 bp 1.0 81.2