Hotspot

ndhK-ndhC

LSC · 53523-53586 · 64 bp

Pi 0.0312

MarkerSeek score 70.50

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 2
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	AGAGAAGCGGCCATAAACTG	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	211 bp	1.0	79.9
ndhK-ndhC_p2	AGAGAAGCGGCCATAAACTG	GGGCGATGAGTTTCGATGTA	50.0	50.0	58.0	57.8	298 bp	1.0	78.5
ndhK-ndhC_p3	ACCATAGAGAAGCGGCCATA	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.3	58.1	216 bp	1.0	77.8
ndhK-ndhC_p4	ACCATAGAGAAGCGGCCATA	GGGCGATGAGTTTCGATGTA	50.0	50.0	58.3	57.8	303 bp	1.0	76.5
ndhK-ndhC_p5	CCATAGAGAAGCGGCCATAA	CATGGCGAAAAGGAGCATTG	50.0	50.0	57.2	58.1	215 bp	1.0	73.4