Hotspot

ycf3-trnS-GGA

LSC · 47428-48286 · 859 bp

Back to Beesia

Pi 0.0223
MarkerSeek score 72.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
859
variable_sites
19
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 945-948 bp 1.0 73.1
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 966-969 bp 1.0 72.6
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 916-919 bp 1.0 72.4
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA GACGGAAAGAGAGGGATTCG 47.6 55.0 58.0 57.8 967-970 bp 1.0 72.1
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 946-949 bp 1.0 70.8