Hotspot

rps14-psaB

LSC · 40206-40334 · 129 bp

Back to Beesia

Pi 0.0388
MarkerSeek score 74.80
Primer available yes
Label rps14-psaB

Feature Metadata

label_name
rps14-psaB
feature_type
igs
region
LSC
length_bp
129
variable_sites
5
pi
0.0388
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14-psaB_p1 GTCACTCAACGACGGAACTT GTGGCTCTATCCATTGTGCA 50.0 50.0 58.2 58.0 360 bp 1.0 79.6
rps14-psaB_p2 GTCACTCAACGACGGAACTT ACTTACGCGGCTTTCTTGAT 50.0 45.0 58.2 57.9 294 bp 1.0 79.4
rps14-psaB_p3 GTCACTCAACGACGGAACTT ATTCACTTACGCGGCTTTCT 50.0 45.0 58.2 57.9 298 bp 1.0 79.4
rps14-psaB_p4 GTCACTCAACGACGGAACTT TTCACTTACGCGGCTTTCTT 50.0 45.0 58.2 58.1 297 bp 1.0 79.1
rps14-psaB_p5 TTTGTCACTCAACGACGGAA GTGGCTCTATCCATTGTGCA 45.0 50.0 57.7 58.0 363 bp 1.0 78.4