Hotspot

psbZ-trnG-GCC

LSC · 39040-39416 · 377 bp

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Pi 0.0647
MarkerSeek score 81.20
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
377
variable_sites
24
pi
0.0647
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTTCTGACGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.4 57.8 534-537 bp 1.0 79.9
psbZ-trnG-GCC_p2 GCTTCTTCTGACGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.9 57.8 532-535 bp 1.0 76.3
psbZ-trnG-GCC_p3 TTGCTTCTTCTGACGGTTGG TACCATTCGACCATATCCGC 50.0 50.0 58.4 57.3 507-510 bp 1.0 75.6
psbZ-trnG-GCC_p4 TTGCTTCTTCTGACGGTTGG TTACCATTCGACCATATCCGC 50.0 47.6 58.4 58.0 508-511 bp 1.0 73.2
psbZ-trnG-GCC_p5 TGCTTCTTCTGACGGTTGG CGTCTTCTCCTTGGCAAAGA 52.6 50.0 57.7 57.8 533-536 bp 1.0 72.7