Hotspot

trnK-UUU-rps16

LSC · 4313-5093 · 781 bp

Back to Beesia

Pi 0.0274
MarkerSeek score 78.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
781
variable_sites
20
pi
0.0274
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 872-909 bp 1.0 82.6
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 871-908 bp 1.0 82.6
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 871-908 bp 1.0 82.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 870-907 bp 1.0 82.6
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGAAAAAGGCGCTCAACCTA 50.0 45.0 58.2 57.7 868-905 bp 1.0 82.3