Hotspot

trnH-GUG-psbA

LSC · 74-373 · 300 bp

Back to Beesia

Pi 0.0947
MarkerSeek score 82.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
300
variable_sites
27
pi
0.0947
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 341-356 bp 1.0 86.3
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 354-369 bp 1.0 86.0
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 355-370 bp 1.0 85.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 356-371 bp 1.0 85.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TGGAAGTTATGCATGAGCGT 55.0 45.0 58.1 57.6 387-402 bp 1.0 84.3