Hotspot

ndhF-trnL-UAG

SSC · 112179-113700 · 1522 bp

Back to Beccarinda

Pi 0.0265
MarkerSeek score 62.80
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1522
variable_sites
40
pi
0.0265
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 AGGAAAGGTATGATCCATGCA TTTAGGAGATGACATGCCGC 42.9 50.0 56.8 58.0 1657-1704 bp 1.0 64.1
ndhF-trnL-UAG_p2 AGGAAAGGTATGATCCATGCA TCCTAAGAGCAGCGTGTCTA 42.9 50.0 56.8 57.9 1602-1649 bp 1.0 63.6
ndhF-trnL-UAG_p3 AGGAAAGGTATGATCCATGCA AGCAGCGTGTCTACCAATTT 42.9 45.0 56.8 57.8 1595-1642 bp 1.0 63.1
ndhF-trnL-UAG_p4 AGGAAAGGTATGATCCATGCA CCTAAGAGCAGCGTGTCTAC 42.9 55.0 56.8 57.8 1601-1648 bp 1.0 63.0
ndhF-trnL-UAG_p5 AGGAAAGGTATGATCCATGCA CTAAGAGCAGCGTGTCTACC 42.9 55.0 56.8 57.8 1600-1647 bp 1.0 63.0