Hotspot

rps11-rpl36

LSC · 80029-80130 · 102 bp

Back to Beccarinda

Pi 0.0297

MarkerSeek score 68.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 102
variable_sites: 3
pi: 0.0297
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GTGCACTCTTACGTGAACCA	AATTTGCTCCAACCCCAGAC	50.0	50.0	58.1	58.4	208-209 bp	1.0	77.5
rps11-rpl36_p2	GTGCACTCTTACGTGAACCA	CGGGGGCGCATTATAGTAAT	50.0	50.0	58.1	57.6	225-226 bp	1.0	77.1
rps11-rpl36_p3	GTGCACTCTTACGTGAACCA	GCTCCAACCCCAGACATAAA	50.0	50.0	58.1	57.5	203-204 bp	1.0	76.4
rps11-rpl36_p4	CGTCCATTCCTACGTGAACC	AATTTGCTCCAACCCCAGAC	55.0	50.0	58.4	58.4	185-186 bp	1.0	75.9
rps11-rpl36_p5	CGTCCATTCCTACGTGAACC	CGGGGGCGCATTATAGTAAT	55.0	50.0	58.4	57.6	202-203 bp	1.0	75.5