Hotspot

petB-petD

LSC · 76890-77805 · 916 bp

Back to Beccarinda

Pi 0.0131
MarkerSeek score 66.30
Primer available yes
Label petB-petD

Feature Metadata

label_name
petB-petD
feature_type
igs
region
LSC
length_bp
916
variable_sites
12
pi
0.0131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB-petD_p1 GCCGCTTCTTACTGCTGTAT ACAGGATCGTTCAAATCAGGT 50.0 42.9 58.1 57.3 1072-1088 bp 1.0 66.8
petB-petD_p2 GCCGCTTCTTACTGCTGTAT ACAGGATCGTTCAAATCAGGTT 50.0 40.9 58.1 58.0 1072-1088 bp 1.0 64.3
petB-petD_p3 ACACACTTTTGTATTGCCGC ACAGGATCGTTCAAATCAGGT 45.0 42.9 57.6 57.3 1087-1103 bp 1.0 63.9
petB-petD_p4 TATTGCCGCTTCTTACTGCT ACAGGATCGTTCAAATCAGGT 45.0 42.9 57.3 57.3 1076-1092 bp 1.0 61.7
petB-petD_p5 CACACTTTTGTATTGCCGCT ACAGGATCGTTCAAATCAGGT 45.0 42.9 57.3 57.3 1086-1102 bp 1.0 61.6