Hotspot

psbH-petB

LSC · 75376-76229 · 854 bp

Back to Beccarinda

Pi 0.0215

MarkerSeek score 72.70

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 18
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TCCTTTGATGGGTGTTGCAA	CGAGACGTTCTTCGAACCAA	45.0	50.0	57.9	58.2	991-1006 bp	1.0	80.1
psbH-petB_p2	TCCTTTGATGGGTGTTGCAA	ATATCATCCGCAATCGCCTG	45.0	50.0	57.9	58.3	1016-1031 bp	1.0	79.8
psbH-petB_p3	CGACTCCTTTGATGGGTGTT	CGAGACGTTCTTCGAACCAA	50.0	50.0	57.8	58.2	995-1010 bp	1.0	79.3
psbH-petB_p4	AACGACTCCTTTGATGGGTG	CGAGACGTTCTTCGAACCAA	50.0	50.0	57.8	58.2	997-1012 bp	1.0	79.2
psbH-petB_p5	CGACTCCTTTGATGGGTGTT	ATATCATCCGCAATCGCCTG	50.0	50.0	57.8	58.3	1020-1035 bp	1.0	78.9