Hotspot

psbE-petL

LSC · 65585-66476 · 892 bp

Back to Beccarinda

Pi 0.0249

MarkerSeek score 72.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 22
pi: 0.0249
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	1007-1013 bp	1.0	73.7
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	1002-1008 bp	1.0	73.4
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.4	57.1	1000-1006 bp	1.0	72.1
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCAA	50.0	47.6	57.8	57.8	1007-1013 bp	1.0	71.3
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGCTCAGACC	50.0	50.0	57.8	56.8	1008-1014 bp	1.0	71.3