Hotspot

ndhC-trnV-UAC

LSC · 50776-51806 · 1031 bp

Back to Beccarinda

Pi 0.0169

MarkerSeek score 67.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 17
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCCGGAAATGAAAAATGCC	AAAAGATGCACGTTGGGTCT	50.0	45.0	58.0	58.0	1202-1223 bp	1.0	82.6
ndhC-trnV-UAC_p2	CCCCGGAAATGAAAAATGCC	AAAGATGCACGTTGGGTCTT	50.0	45.0	58.0	58.0	1201-1222 bp	1.0	82.6
ndhC-trnV-UAC_p3	CCCCGGAAATGAAAAATGCC	AAGATGCACGTTGGGTCTTT	50.0	45.0	58.0	58.0	1200-1221 bp	1.0	82.6
ndhC-trnV-UAC_p4	GGCTAAAACCCCGGAAATGA	AAAGATGCACGTTGGGTCTT	50.0	45.0	58.2	58.0	1209-1230 bp	1.0	81.5
ndhC-trnV-UAC_p5	GGCTAAAACCCCGGAAATGA	AAGATGCACGTTGGGTCTTT	50.0	45.0	58.2	58.0	1208-1229 bp	1.0	81.5