Hotspot

trnF-GAA-ndhJ

LSC · 48426-49093 · 668 bp

Back to Beccarinda

Pi 0.0598

MarkerSeek score 84.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 36
pi: 0.0598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	736-810 bp	1.0	87.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	700-774 bp	1.0	86.8
trnF-GAA-ndhJ_p3	CTACATTGGGAATGGTCGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	753-827 bp	1.0	86.7
trnF-GAA-ndhJ_p4	TGCTACATTGGGAATGGTCG	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	755-829 bp	1.0	86.6
trnF-GAA-ndhJ_p5	GGATGGGATGCTACATTGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.1	58.1	763-837 bp	1.0	86.2