Hotspot

trnT-UGU-trnL-UAA

LSC · 46800-47430 · 631 bp

Back to Beccarinda

Pi 0.0351

MarkerSeek score 65.00

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 22
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	TTTTCCGGCGATACGAATGT	GCTTCCATTGAGTCTCTGCA	45.0	50.0	58.0	57.9	1531-1558 bp	1.0	81.1
trnT-UGU-trnL-UAA_p2	TTTTCCGGCGATACGAATGT	TTTTCGCTCTCTGAACGCTT	45.0	45.0	58.0	58.1	1501-1528 bp	1.0	80.9
trnT-UGU-trnL-UAA_p3	TTTTCCGGCGATACGAATGT	TTTTTCGCTCTCTGAACGCT	45.0	45.0	58.0	58.1	1502-1529 bp	1.0	80.9
trnT-UGU-trnL-UAA_p4	AAACTTGACCTGTTGACCCC	GCTTCCATTGAGTCTCTGCA	50.0	50.0	57.9	57.9	1556-1583 bp	1.0	80.6
trnT-UGU-trnL-UAA_p5	AAACTTGACCTGTTGACCCC	TTTTCGCTCTCTGAACGCTT	50.0	45.0	57.9	58.1	1526-1553 bp	1.0	80.4