Hotspot

trnS-GCU-trnS-CGA

LSC · 8140-8888 · 749 bp

Back to Beccarinda

Pi 0.0275

MarkerSeek score 62.20

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 20
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	804 bp	0.5	41.1
trnS-GCU-trnS-CGA_p2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	803 bp	0.5	41.1
trnS-GCU-trnS-CGA_p3	ACGGAAAGAGAGGGATTCGA	ACCACTAAACTATACCCGCT	50.0	45.0	58.2	55.6	858 bp	0.5	40.0
trnS-GCU-trnS-CGA_p4	AGCAATCCGACGCTTTAGTC	ACCACTAAACTATACCCGCT	50.0	45.0	58.4	55.6	806 bp	0.5	38.3
trnS-GCU-trnS-CGA_p5	CGACGCTTTAGTCCACTCAG	ACCACTAAACTATACCCGCT	55.0	45.0	58.4	55.6	799 bp	0.5	38.1