Hotspot

ycf1

IRb · 111332-112315 · 984 bp

Back to Bauhinia

Pi 0.0451

MarkerSeek score 67.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 984
variable_sites: 128
pi: 0.0451
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	TATGTAGGAGGTGGCCGAAT	55.0	50.0	58.0	57.9	1161-1616 bp	1.0	91.2
ycf1_p2	GAGCTACTGAGGAACAACGG	ACTTTCTTCGTTGGCACCAT	55.0	45.0	58.0	58.0	1620-2084 bp	0.875	86.8
ycf1_p3	TAACTCGCGGAACTTCTTCG	ACTTTCTTCGTTGGCACCAT	50.0	45.0	58.0	58.0	1871-1994 bp	0.75	78.0
ycf1_p4	GTATCCCGGACCTACACTGA	ACTTTCTTCGTTGGCACCAT	55.0	45.0	57.9	58.0	2262-2317 bp	0.5	65.1
ycf1_p5	AGTATCCCGGACCTACACTG	ACTTTCTTCGTTGGCACCAT	55.0	45.0	57.9	58.0	2263-2318 bp	0.5	65.0