Hotspot

ycf2-trnI-CAU

IRa · 154120-154426 · 307 bp

Back to Bauhinia

Pi 0.158

MarkerSeek score 57.10

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 307
variable_sites: 99
pi: 0.158
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	906-1135 bp	1.0	91.7
ycf2-trnI-CAU_p2	AATCATGGTCCTTGCGGATC	ACCGGGAGTCGATGACTATT	50.0	50.0	58.0	57.9	1303-1517 bp	1.0	90.6
ycf2-trnI-CAU_p3	AATCATGGTCCTTGCGGATC	AGAAGAGTTCGACCCAATGC	50.0	50.0	58.0	57.9	1260-1474 bp	1.0	90.6
ycf2-trnI-CAU_p4	AATCATGGTCCTTGCGGATC	GAAGAGTTCGACCCAATGCT	50.0	50.0	58.0	57.9	1259-1473 bp	1.0	90.6
ycf2-trnI-CAU_p5	AATCATGGTCCTTGCGGATC	GACTGTCTACGGCTCCATTG	50.0	55.0	58.0	58.1	1491-1802 bp	1.0	90.3