Hotspot

rpl32-trnL-UAG

SSC · 115831-116188 · 358 bp

Back to Bauhinia

Pi 0.051

MarkerSeek score 70.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 358
variable_sites: 62
pi: 0.051
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGGTTGGGTTCGTAAACTT	ACTCGGTGCGATTATTGTCC	50.0	50.0	58.0	58.1	1335-1360 bp	0.875	79.9
rpl32-trnL-UAG_p2	GGATATTGGGCAGCATGGAA	ACTCGGTGCGATTATTGTCC	50.0	50.0	58.0	58.1	973 bp	0.25	49.5
rpl32-trnL-UAG_p3	AAGGGATATTGGGCAGCATG	ACTCGGTGCGATTATTGTCC	50.0	50.0	58.0	58.1	976 bp	0.25	49.4
rpl32-trnL-UAG_p4	GGATATTGGGCAGCATGGAA	GCTGCATAGCCCAATACCAT	50.0	50.0	58.0	58.1	1104 bp	0.25	49.3
rpl32-trnL-UAG_p5	AAGGGATATTGGGCAGCATG	GCTGCATAGCCCAATACCAT	50.0	50.0	58.0	58.1	1107 bp	0.25	49.3