Hotspot

ycf1-ndhF

SSC · 112316-112386 · 71 bp

Back to Bauhinia

Pi 0.163

MarkerSeek score 69.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 71
variable_sites: 29
pi: 0.163
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GATATCCGCCGAAGAAGACC	ACTTTCTTCGTTGGCACCAT	55.0	45.0	57.9	58.0	804 bp	0.25	49.3
ycf1-ndhF_p2	AGCGGTCAAATTGAGAAGCA	ACTTTCTTCGTTGGCACCAT	45.0	45.0	58.1	58.0	836 bp	0.25	49.0
ycf1-ndhF_p3	GATATCCGCCGAAGAAGACC	AATATGTAGGAGGTGGCCGA	55.0	50.0	57.9	57.9	338 bp	0.25	48.7
ycf1-ndhF_p4	GATATCCGCCGAAGAAGACC	ATATGTAGGAGGTGGCCGAA	55.0	50.0	57.9	57.9	337 bp	0.25	48.7
ycf1-ndhF_p5	GATATCCGCCGAAGAAGACC	TATGTAGGAGGTGGCCGAAT	55.0	50.0	57.9	57.9	336 bp	0.25	48.7