Hotspot

psbZ-trnG-GCC

LSC · 37896-38367 · 472 bp

Back to Bauhinia

Pi 0.0493
MarkerSeek score 72.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
472
variable_sites
78
pi
0.0493
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CAACCACTCAGCCATCTCTC CTTGAGGTCACGGGTTCAAA 55.0 50.0 58.0 58.0 1299-1401 bp 1.0 90.7
psbZ-trnG-GCC_p2 CAACCACTCAGCCATCTCTC CTTGTGTTTATCGCGGGGTA 55.0 50.0 58.0 58.0 1350-1408 bp 0.875 81.9
psbZ-trnG-GCC_p3 CAACCACTCAGCCATCTCTC TTGTGTTTATCGCGGGGTAG 55.0 50.0 58.0 58.0 1349-1407 bp 0.875 81.9
psbZ-trnG-GCC_p4 CAACCACTCAGCCATCTCTC CGATCATAGGAGGGTCCCTT 55.0 55.0 58.0 58.0 1458-1516 bp 0.875 81.8
psbZ-trnG-GCC_p5 CAACCACTCAGCCATCTCTC CGTTGTTTTTCGACCGGAAG 55.0 50.0 58.0 58.0 1622-1680 bp 0.875 81.5