Hotspot

trnR-UCU-atpA

LSC · 10137-10530 · 394 bp

Back to Bauhinia

Pi 0.0478

MarkerSeek score 71.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 394
variable_sites: 54
pi: 0.0478
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CCCGCTACCCGCTTATATTC	TGAGTTGCTCAAACAGTCCC	55.0	50.0	58.0	58.0	992-1036 bp	1.0	91.4
trnR-UCU-atpA_p2	CCCGCTACCCGCTTATATTC	GAGTTGCTCAAACAGTCCCA	55.0	50.0	58.0	58.0	991-1035 bp	1.0	91.3
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	TGAGTTGCTCAAACAGTCCC	50.0	50.0	58.0	58.0	1023-1067 bp	1.0	91.0
trnR-UCU-atpA_p4	AGCCTTCCAAGCTAACGATG	GAGTTGCTCAAACAGTCCCA	50.0	50.0	58.0	58.0	1022-1066 bp	1.0	90.9
trnR-UCU-atpA_p5	CCCGCTACCCGCTTATATTC	CGAAGCTTATCCGGGAGATG	55.0	55.0	58.0	57.9	1394-1438 bp	1.0	89.3