Hotspot

trnS-GCU-trnG-UCC

LSC · 8455-9062 · 608 bp

Back to Bauhinia

Pi 0.0409

MarkerSeek score 71.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 79
pi: 0.0409
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	57.9	45.0	58.1	54.0	634-811 bp	1.0	52.0
trnS-GCU-trnG-UCC_p2	TCCACTCAGCCATCTCTCC	TTACCACTAAACTATACCCGC	57.9	42.9	58.1	54.9	635-812 bp	1.0	52.0
trnS-GCU-trnG-UCC_p3	TCCACTCAGCCATCTCTCC	TTTACCACTAAACTATACCCGC	57.9	40.9	58.1	55.6	636-813 bp	1.0	52.0
trnS-GCU-trnG-UCC_p4	TCCACTCAGCCATCTCTCC	CTTTTACCACTAAACTATACCCGC	57.9	41.7	58.1	57.4	638-815 bp	1.0	52.0
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	TTTTACCACTAAACTATACCCGC	57.9	39.1	58.1	56.4	637-814 bp	1.0	52.0